The WBC, also known as leukocytes, is a major component of the immune system.

 It protects the body from infectious microorganisms and foreign intruders. There are certain categories of WBC that are generated from the hematopoietic stem cells of bone marrow. 

The WBCs are found throughout the body due to its lymphatic structure [4,5]. During the disease screening process, the WBC/leukocyte count plays a vital role. 

This count is normally measured using the universal unit “cells per microliter (cells/mcL)” where the normal count ranges from 4,000 to 11,000 cells/mcL YICHANG. A decrease in the value indicates a lower immunity level, and an increase presents the possibility of infection. 

As per the literature, there are five WBC categories present in the human body, each having its own function and texture pattern TPE gloves. The clinical-level recording of the WBC is achieved using digital microscopes. The recorded microscopic image is then assessed using a suitable WBC detection system Synthetic Gloves. Various WBC cells prepared from thin blood smear viewed under a digital microscope are presented in Figure 1.4. 


 

These sample images were collected from the benchmark database known as Leukocyte Images for Segmentation and Classification (LISC) [6,7]. The thin blood smear images of WBCs in Figure 1.4 (a) to (e) are the Basophil, Eosinophil, Lymphocyte, Monocyte and Neutrophil. Figure 1.4 (f) presents the image of a mixed WBC recorded using a thin blood smear.

 During the image acquisition process, blood samples collected from the peripheral blood of 8 healthy and 400 infected patients are smeared on the slides and viewed under a microscope. 

These slides are smeared with Gismo-Right technique and viewed with a light microscope (Microscope-Axioskope 40) using an achromatic lens with a magnification factor of 100. 

The view fields are then captured by a digital camera (Sony Model No. SSCDC50AP) and are saved as .bmp files with a pixel resolution of 720 × 576 × 3. The earlier research work carried out using the LISC data can be found in [7].

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